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Fig. 3. Immunohistochemistry of P2 neonatal (A-C) and adult (D) mouse brain. All sections oriented with dorsal up, lateral to the left; S, striatum; asterisk (*), lateral ventricle. Owing to fixation, tissue processing and dehydration, the ventricle is closed in A-C. Coronal sections show prominent staining of the ependymal layer lining the lateral ventricle with anti-dynein 2 HC (A,D), anti-LIC3 (B), anti-CGI53 IFT-particle (C) Abs. Cells outside the ependymal layer are also weakly stained. Dynein 2 HC is observed in the ependymal layer of both neonate and adult. Bar, 100 µm.