Fig. 4. In situ hybridization of dynein 1 and 2, SCG10 in mammalian nervous system. (A-D) Cross-section of fetal mouse (E12.5) neural tube at the lumbar level is hybridized with dynein 2 HC antisense (A), dynein 2 HC sense (B), dynein 1 HC antisense (C), or SCG 10 antisense (D) probes. (A) Dynein 2 staining is most intense in the ependymal layer, with some additional staining observed in the mantle layer. (C) Dynein 1 in contrast, is restricted to the mantle layer in addition to the apical regions of ependymal cells lining the central canal. Note overall dynein 2 HC mRNA level is much lower than that of dynein 1 (staining time 16 hours in dynein 1 vs. 48 hours in dynein 2). Bar, 100 µm (A). (E-H) mRNA localization in juvenile mouse (P2) olfactory epithelium. Sections oriented with dorsal up. At low magnification dynein 2 HC (E), dynein 1 HC (F), and SCG10 (G) staining is concentrated in the epithelial layer. At higher magnification (H), they show different patterns of distribution. Dynein 2 mRNA is expressed in the basal (BA) and receptor cells (RE), whereas dynein 1 is expressed only in the receptor cells. SCG10 is confined in approximately the basal half population of the receptor cells. In the supporting cells (SP), both dynein 2 and dynein 1 are observed but SCG10 is absent. Bar, 200 µm (E-G); 25 µm (H).