Fig. 5. Comparison of the effects of Wnts and Ihh on chondrocyte terminal differentiation of stage 23/24 wing mesenchymal cells in micromass cultures after seven days. (A) Quantification of alkaline phosphatase activity. (B) Micromass cultures stained for alkaline phosphatase activity (red staining). (C) Micromass cultures stained for type X collagen (red fluorescence; magnification x10); examples of the stained nodules are outlined. In A, values shown are the mean+s.d. of nine cultures from three independent experiments. ^*P<0.005; ^**P<0.001 (Student's t-test) of Wnt/Ihh-infected cultures compared with control cultures.