Fig. 7. Effect of misexpression of Ihh in vivo. Chick embryonic fibroblasts (CEFs) infected with retrovirus were grafted into the stage 18/19 wing bud. Limbs were fixed after a further 7 days (i.e. at embryonic day 10) and processed for in situ hybridisation. A,C,E and B,D,F are adjacent sections from two different embryos. Light field (A,B) and dark field (C-F) micrographs showing endogenous and misexpressed Ihh (C,D) and the effect of Ihh on the expression of Wnt5b (E) and Wnt11 (F). The inset in A shows a high power view of the chondrocytes. Bars, 500 µm (A,B); 200 µm (inset).