Fig. 3. Schematic representation of chimeric photoproteins (CgA fragment-GFP fusion proteins) designed to study CgA trafficking to the dense core secretory granules of PC12 cells. Construction of plasmids encoding GFP fusion proteins was performed as described in Materials and Methods. Plasmid numbering refers to the position of the 3' end of the CgA fragment subcloned, within the original human CgA cDNA (GenBank NM_001275). For example, in pCMV-CgA805-EGFP, the 3' end of the subcloned CgA fragment (at amino acid +224) is located at 805 bp in the original cDNA. Amino acid residue positions are indicated by numbers alongside the chimera. Proteins are drawn proportional to scale.