Fig. 4. Immunochemical detection of the expression of GFP fusion proteins in PC12 cells. Total PC12 cell lysates were prepared 72 hours after transfection with expression plasmids encoding the GFP fusion proteins indicated. Expression of GFP fusion proteins was evaluated by SDS-PAGE followed by immunoblotting using an anti-GFP antibody. Immunoreactivity was visualized by chemiluminescence. Estimation of size (M_m, calculated molecular mass, based upon primary structure of the chimera; M_a, apparent molecular mass by SDS-PAGE): SgP-CgA_1-16-GFP (28.6 kDa; 27 kDa); SgP-CgA_1-37-GFP (30.9 kDa; 29.2 kDa); SgP-CgA_1-39-GFP (31.2 kDa; 29.5 kDa); SgP-CgA_1-77-GFP (35.6 kDa; 35 kDa); SgP-CgA_1-115-GFP (39.9 kDa; 40 kDa); SgP-CgA_1-153-GFP (43.8 kDa; 48.5 kDa); SgP-CgA_1-168-GFP (45.6 kDa; 52.5 kDa); SgP-CgA_1-209-GFP (49.9 kDa; 62 kDa); SgP-CgA_1-224-GFP (51.6 kDa; 71 kDa); SgP-CgA-GFP (75.8 kDa; 105 kDa); SgP-CgAC₁₇E-GFP (75.8 kDa; 105 kDa); SgP-CgA_233-439-GFP (50.3 kDa; 56 kDa).