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Fig. 5. Subcellular distribution of the CgA fragment-GFP chimeric photoproteins in PC12 cells. 48 hours after transfection with expression plasmids encoding the GFP fusion proteins indicated, aldehyde-fixed cells were examined by deconvolution microscopy. GFP was excited at {lambda}ex 490±10 nm and imaged at {lambda}em 528±238 nm. Nuclei were visualized with (blue) Hoechst 33342 ({lambda}ex 350 nm/{lambda}em 461 nm). Optical sections along the Z axis were acquired with increments of 0.2 µm using 60x or 100x oil immersion objectives (1.4 NA). Data were processed to generate 3D/volume views of the GFP chimera distributions. Bars, 5 µm.