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Fig. 6. I{kappa}B{alpha}AS enhances NF{kappa}B binding activity following UV, TNF-{alpha}, pervanadate or H/R treatment. HeLa cells were infected with either Ad.BglII or Ad.I{kappa}B{alpha}AS at an moi of 1000 particles/cell for 24 hours. Cells were treated with (A) UV (50 J/m2), (B) TNF-{alpha} (10 ng/ml), (C) pervanadate (50 µM) or (D) H/R (5 hours hypoxia, 6 hours reoxygenation). Both treated and untreated cells were collected for nuclear extract preparation and EMSA analysis. Ad.I{kappa}B{alpha}AS-infected cells demonstrated a higher baseline of NF{kappa}B DNA-binding activity (lanes 1 versus lane 4) and enhanced NF{kappa}B activation following all treatments compared with Ad BglII-infected cells (lanes 2,3 versus lanes 5,6). The conditions for various experimental groups are marked above each panel.