JCS -- Fan et al. 115 (24): 4843 Figure IG7

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 7. I ${kappa}$ B ${alpha}$ AS enhances NF ${kappa}$ B transcriptional activity following UV, TNF- ${alpha}$ , pervanadate or H/R treatment. HeLa cells were co-infected with Ad.NF ${kappa}$ Bluc (500 particles/cell) together with either Ad.BglII or Ad.I ${kappa}$ B ${alpha}$ AS at an moi of 1000 particles/cell for 24 hours. Cells were treated with (A) UV (50 J/m²), (B) TNF- ${alpha}$ (10 ng/ml), (C) pervanadate (50 µM) and (D) H/R (5 hours hypoxia, 6 hours reoxygenation). Luciferase assays were performed on samples harvested 6 hours after treatment. Results depict the mean (±s.e.m., n=6) raw unadjusted relative light units (RLU). NF ${kappa}$ B transcriptional activity was significantly enhanced (P<0.01) in the Ad.I ${kappa}$ B ${alpha}$ AS infected groups, as compared to the groups infected by Ad.BglII, following UV, TNF- ${alpha}$ , pervanadate or H/R treatment.