Fig. 3. Cortactin and arp2/3 complex accumulation were abnormal on individualization complexes in myosin VI mutants. (A-L) Low magnification of a confocal image of wild-type (A-C, G-I) or myosin VI mutant (jar/jar; D-F, J-L) individualization complexes stained with Alexa Fluor 488 phalloidin (A,D,G,J) and anti-cortactin antibodies (B,E) or anti-arp3 antibodies (H,K). (M-X) Zoomed confocal images of a few actin cones from the individualization complexes in A-L. Cortactin localized to the fronts of the actin cones (blue arrow; B,N) and on the cyst membrane (yellow arrow; B). Cortactin localized to cyst membranes (yellow arrow, E) and actin cones in mutants but was not concentrated at the front of the actin cones (white arrow; E,Q). Cortactin localized normally to the cyst membrane in the myosin VI mutant (yellow arrow, E). Arp3 localized to the front of actin cones (blue arrowhead; H,T) and the cytoplasm of the cystic bulge (H). Arp3 was present on actin cones in mutants but was not well concentrated at the front of actin cones (white arrowhead; K,W). Images of wild-type and myosin VI mutant testes were collected at the same gain.