Fig. 5. Actin stability defects in dynamin myosin VI double mutants. Confocal projections of mutant testes stained with phalloidin. Volume samples were taken near the basal end of testis where all the actin individualization complexes are associated with nuclei. Within each testis, several groups of 64 bundled spermatids are shown. (A) All actin individualization complexes in dynamin (shi¹/Y) single mutants exposed to the non-permissive temperature for 6 hours stained strongly with phalloidin (white arrowhead). (B) Most actin complexes in myosin VI single mutants (jar/jar) exposed to the non-permissive temperature for 6 hours stained brightly with phalloidin, although a few disrupted complexes stained weakly with phalloidin (asterisk). (C) Few actin complexes in dynamin myosin VI double mutants (shi¹/Y; jar/jar) exposed to the non-permissive temperature for 6 hours were visible, and those that were visible stained weakly with phalloidin (white arrow). All images are projections of multiple planes. Images of single and double mutants were taken at the same gain and scale.