Fig. 3. Neurite outgrowth from explants plated on neutral or acidic laminin matrices. Brain explants were incubated for 24 hours on laminin matrices obtained at neutral (A,B) or acidic pH (C,D). Explants were developed for the neuronal marker ß tubulin III (A,C) and for total cells, using DAPI (B,D). Arrows point to typical aggregates of cell bodies occurring on the neutral matrix. The inset in A shows short neurites emerging from a rare region of the explant where aggregation of cell bodies did not occur. Bar, 100 µm.