Fig. 6. Double staining for laminin and neuronal cells. Cortical cells were plated over laminin polymerized in neutral buffer. After 24 hours immunocytochemical analyses for laminin (rabbit polyclonal) and for the neuronal marker, ß tubulin (mouse monoclonal), were performed. A shows the laminin matrix and B neuronal cell bodies accumulating on regions corresponding to large laminin aggregates. The scale bar corresponds to 50 µm.