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Fig. 7. BrdU incorporation by cells plated on neutral or acidic laminin matrices. Neurons were plated on neutral (A, C and E) or acidic laminin matrices (B, D and F). After 2 hours BrdU was added and kept in culture for the following 22 hours, after which cells were fixed, permeabilized and analyzed for the incorporation of BrdU. A and B show cells that incorporated BrdU, C and D show the same fields analyzed for the nuclear marker, DAPI and E and F show the BrdU and DAPI images overlaid using the software Photoshop. Bar, 100 µm. G represents the quantitative analysis of data in panels A to F. One asterisk indicates P<0.05.