Fig. 5. (A) Kinetics of ABI1 and ABI2 expression. The time-dependent expression of ABI1 and ABI2 in the presence and absence of ABA in WT and abi1-1 was monitored at the indicated time points. The 25S RNA band stained with ethidium bromide served as a loading control of the RNA gels. (B) Subcellular localization of ABI1 and abi1. GFP fluorescence of ABI1-GFP (left), abi1-GFP (middle) and GFP (right) in onion epidermal cells was observed 16 hours after biolistic bombardment. Representative cells are shown. Bars, 50 µm.