Fig. 10. Myr-Chat expression enhances the cell adhesion on fibronectin. 293T cells were transfected with a control pCAX or indicated Chat variant plasmid together with pCAX-EGFP. Cell adhesion on 96-well culture plates coated with 0.2 µg/ml fibronectin was measured as described in the Materials and Methods. Mean values obtained from the average of six independent wells are shown with s.d. The asterisk indicates the value with a statistically significant increase (P<0.01) over control cells determined by Student's t-test. Control and Chat-transfected cells adhered to the fibronectin matrix to a similar extent. Myr-Chat-expressing cells showed a 1.5 times increase of adhesion compared with control cells. Expression of Myr-CT did not enhance the adhesion.