Fig. 2. DRAL/FHL-2 colocalises with the N2B region of titin. (A) Schematic representation of antibody epitopes on titin. (B) Mapping of the exact position of DRAL/FHL-2 in the I-band by costaining with antibodies against different titin epitopes. Cardiac myofibrils from papillary muscle (a,b,c) or neonatal rat cardiomyocytes (d) were stained with an antibody directed against DRAL/FHL-2 (a', b', c', d'), together with antibodies directed against the N-terminus of titin (T12 epitope, a), the N2B region (I19 epitope, b), the N2A region (c) and against the PEVK region (9D10 epitope, d). DRAL/FHL-2 colocalises with the N2B region, but not with the other regions of titin. Arrowheads indicate the center of the Z-disc. Bar, 2 µm.