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Fig. 3. DRAL/FHL-2 interacts with the N2B region of titin in a yeast two-hybrid assay. Yeast strain L40 was cotransformed with LexA-DRAL/FHL-2 and Gal4AD-N2B (lane 1), LexA-lamin C and Gal4AD-N2B (lane 2), LexA and Gal4AD-N2B (lane 3) and LexA-DRAL/FHL-2 and Gal4AD (lane 4). Yeast transformants were streaked out on selective plates (a) and ß-galactosidase activity was detected using a filter assay (b). Results of two independent colonies per yeast transformation are shown. Only LexA-DRAL/FHL-2 in combination with Gal4AD-N2B yields any measurable ß-galactosidase activity (lane 1). N2B does not interact with an unrelated bait (LexA-lamin C, lane 2) or with LexA alone (lane 3) and LexA-DRAL/FHL-2 does not interact with Gal4AD alone (lane 4).