Fig. 2. Co-transfections of wild-type and variant rNFL with rNFM or rNFH in SW13Vim^- cells. (A-D) Rat NFM was co-transfected with wild-type rNFL (A,B) or D469N variant rNFL (C,D). The cells were double-labeled with polyclonal anti-NFL antibody (A,C) and monoclonal anti-NFM antibody (B,D). For both wild-type and variant NFL, a filamentous network throughout the cytoplasm was observed that stained with both antibodies. (E-H) Rat NFH was co-transfected with wild-type rNFL (E,F) or D469N variant rNFL (G,H). The cells were double-labeled with polyclonal anti-NFL antibody (E,G) and monoclonal anti-NFH antibody (F,H). A filamentous network was formed with both wild-type and variant rNFL co-transfected with rNFH. In all the subsequent experiments, the variant NFL behaved identically to wild-type NFL. Bar, 25 µm.