Fig. 3. Co-transfections of mutant rNFL with rNFM or rNFH in SW13 Vim^- cells. (A-F) Rat NFM was co-transfected with P8R mutant rNFL (A-D) and Q333P mutant rNFL (E,F). The cells were double-labeled with polyclonal anti-NFL antibody (A,C,E) and monoclonal anti-NFM antibody (B,D,F). The P8R mutant rNFL co-transfected with rNFM either formed a bundle of filaments (A,B) or displayed punctate or aggregate staining (C,D). The Q333P mutant rNFL resulted in aggregates that contained rNFM (E,F). (G-L) Rat NFH was co-transfected with P8R mutant rNFL (G-J) and Q333P mutant rNFL (K,L). The cells were double-labeled with polyclonal anti-NFL antibody (G,I,K) and monoclonal anti-NFH antibody (H,J,L). The P8R mutant co-assembled into bundled filaments with rNFH in most cases (G,H), or into more punctate material or aggregates (I,J), whereas the Q333P mutant rNFL resulted only in the formation of aggregates with rNFH (K,L). Bar, 25 µm.