Fig. 5. Co-transfection of hNFL constructs with hNFM in SW13 Vim^- cells. hNFM was co-transfected with either wild-type hNFL (A,B), P8R mutant hNFL (C,D) and Q333P mutant hNFL (E,F). The cells were double-labeled with polyclonal anti-NFL (A,C,E) and monoclonal anti-NFM (B,D,F) antibodies. A filamentous network throughout the cytoplasm was observed for wild-type hNFL and hNFM (A,B). The P8R mutant hNFL formed bundled filaments that stayed on one side of the cell (C,D; cell on the left) or around the nucleus (C,D; cell on the right). The Q333P mutant NFL always formed aggregates with hNFM (E,F). Bar, 25 µm.