Fig. 7. Analysis of the interacting domain of Sbh1p with Cnx1p using the two-hybrid
system. All diploids are able to grow on medium A, and only interaction
between Sbh1p and Cnx1p allows growth on medium B (expression of two reporter
genes is required). 1 and 2, pAS2
SBH1 +
pGADCNX1 (entire protein); 3,
pAS2
SBH1
SalI (deletion of the
C-terminal domain) + pGADCNX1; 4, pAS2
+
pGADCNX1.