Fig. 3. GFP-Yck2p deletion mutants. (A) Illustration of full length GFP-Yck2 fusion protein, C-terminal substitution mutants (Cys^545,546Ser; CIIS), C-terminal truncation mutant, C-terminal deletion mutant with intact -Cys-Cys motif, catalytic domain deletion mutant, and consecutive smaller C-terminal deletions. GFP portions are shown in green and Yck2p portions in gray. Plasma membrane localization (+) or mislocalization (-) is indicated to the right of each fusion protein. Color coding for smaller deletions matches that in panel B. (B) Yck2p C-terminal amino acid sequence with deleted regions indicated as follows: yck2374-390p in red; yck2391-412p in yellow, yck2406-409p with underscore; yck2413-444p in green; yck2445-470p in dark blue; yck2471-498p in violet; yck2499-518p in light blue; yck2519-527p in orange; yck2528-540p in turquoise.