Fig. 4. Localization of Yck2p C-terminal variants in sec mutants at permissive and restrictive temperatures. Cultures of strains LRB936 (sec18) and LRB934 (sec9) carrying pGAL1 plasmids expressing the indicated Yck2p variants were grown and treated as described for Fig. 2, except that the induction period was 120 minutes before images were captured. Bar, 2 µm.