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Fig. 5. Paracellular resistance (Rpara) of MDCK monolayers as derived from transepithelial impedance analysis. In monolayers of C7-cld-2 (transfected with claudin-2 cDNA), Rpara was lower (P<0.001) than in clone C7-vec (transfected with vector only), although higher (P<0.001) than in MDCK clone C11-vec. With low Ca2+ Ringer solution, Rpara decreased in the C7 clones. In C11-vec exposed to high Ca2+ Ringer's, Rpara increased.