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Fig. 3. Distribution of Lte1 in polarity mutants. (A) Localisation of Lte1GFP in a cdc28-13 strain (SY127) at the permissive temperature and after incubation at 37°C. (B) Haploid cdc42-1 cells expressing either endogenous Lte1GFP (SY128, upper panel) or Cdc24GFP (SY129, bottom panel) were examined at the permissive temperature and 2 hours after shift to 37°C. (C) Haploid gic1{Delta}gic2{Delta} cells expressing endogenous Lte1GFP (SY151) were shifted to 37°C for 3 hours and Lte1 localisation monitored by microscopy. (D) Wild-type (SY125), bni1{Delta} (SY130) and bud6{Delta} (SY131) cells were released from their block in G0 at 30°C and analysed for Lte1 localisation after 2 hours. (A-D) Numbers indicate the percentage of cells with shown localisation pattern.