Fig. 1. Acanthamoeba expression vectors. (A) pUb vector has an Acanthamoeba ubiquitin promoter (Ubp). DNAs for EGFP fusion proteins were inserted at NcoI and XbaI restriction sites between the promoter and the poly A signal. (B) Coding sequences for expression. PI, profilin I; MIIt, C-terminal 256 residues of myosin-II tail; MII, full length myosin-II; MIIm, full length myosin-II mutated at three phosphorylation sites to alanine; MIC, myosin-IC (b, basic region; g1, GPA1 domain; s, SH3 domain; and g2, GPA2 domain). Three phosphorylatable serines (S) in the tail piece of pUbGMIIt and pUbGMII were replaced by three alanines (A) in pUbGMIIm.