Fig. 10. Time sequences of fluorescence micrographs of two flattened cells expressing EGFP-myosin-IC. Fluorescence concentrates around a contractile vacuole (CV, black asterisk) just prior to contraction and macropinocytic cups (white asterisk) just before closure. The series of white asterisks trace the ingestion of medium by a macropinocytic cup (MPC). Time is in seconds. Bar, 10 µm.