Fig. 11. Behavior of EGFP-myosin-IC truncation mutants. Fluorescence micrographs of flattened cells expressing EGFP fusions with truncated myosin-IC constructs taken 1 second before contraction of the contractile vacuoles (CV, black asterisks) and closure of the macropinocytic cups (MPC, white asterisks). MICs lacks the C-terminal GPA2 region (residues I1051 to M1186). EGFP-MICg lacks the C-terminal SH3 and GPA2 region (residues P996 to M1186). EGFP-MICb lacks the C-terminal GPA and SH3 domains (residues L940 to M1186). EGFP-MICh lacks the entire tail (residues N720 to M1186). Constructs with the head, basic, GPA1 and SH3 domains concentrated around the contractile vacuoles and macropinocytic cups. Constructs lacking the SH3 domain distributed diffusely in the cytoplasm. Bar, 10 µm.