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Fig. 4. Fluorescence micrographs of live amoebas expressing (A,B) profilin-I-EGFP and (C,D) EGFP. (A,B) Profilin-I-EGFP fills the cytoplasm and concentrates in pseudopods. (C,D) EGFP not only fills the cytoplasm but concentrates in nuclei except for the nucleolus. Bar, 10 µm.