Fig. 8. Distribution of myosin-II filaments in a live, flattened amoeba expressing constitutively active EGFP-full length myosin-II with alanine substituted for serine at all three heavy chain phosphorylation sites. The leading edge is to the right. Fluorescence micrographs at (A) time zero and (B) 21 seconds. Fluorescence spots concentrated at the leading and trailing edge but also clustered around vesicles of various sizes. Two vesicles fused between these two time intervals (arrows). Bar, 10 µm.