Fig. 2. Cloning the p54 cDNA. (A) Schematic representation of the p54 cDNA and the cloning strategy by 5' and 3' RACE (see Materials and Methods for details). SL denotes the spliced leader sequence, UAP is a universal amplification primer. In frame stop codons are depicted by asterisks. (B) Deduced amino acid sequence of Crithidia p54. Amino acids identified from direct peptide sequencing are underlined. The cDNA and amino acid sequences have been deposited in GenBank under accession no. AJ494838. (C) Schematic representation of the Crithidia p54 (CfNek) domain structure in comparison to the original NIMA protein from Aspergillus nidulans.