Fig. 10. Hyperbranching after washing out inhibitors of IP₃ receptor and PLC. (A) Within 20 minutes of washing out 25 µM 2-APB, hyphal widening, which extended over 20-40 µm from the tip, and an apical hyperbranching phenotype was observed. (B) U-73122 (10 µM) wash-out also caused hyphal widening and multiple apical branches. The picture was taken 14 minutes after wash out. (C) Control hypha 16 minutes after wash out. Hyperbranching was not observed after wash-out of cyclopiazonic acid (not shown); in this case, hypha resumed normal hyphal elongation, similar to C. Bars, 10 µm.