Fig. 8. Effects of PLC inhibitors on hyphal growth in N. crassa. (A) U-73122 (400 µM) causes complete inhibition. (B) Control compound U-73343 did not affect hyphal elongation and morphology. (C) 3-nitrocoumarin (20 µg/ml) inhibited hyphal growth. The hypha recovered and restarted growth after washing out the inhibitor at 10 minutes. Pictures were taken at the time shown. Time 0 represents the addition of the inhibitor. Bars, 10 µm.