Fig. 2. Effects of detergent extraction on COS7 cells. (A-C) Scanning electron microscopy of cells extracted in 0.6% saponin for 15 minutes (A) or 30 minutes (B,C). Area boxed in B is shown at higher magnification in C. (D-F) Immunofluorescence staining of cells fixed without prior extraction (D) or cells extracted with saponin for 30 minutes (E,F). Green fluorescence: anti-ß tubulin (D,E); anti-vimentin (F). Red fluorescence: phalloidin (D-F). Purple fluorescence: nuclei stained with TOTO-3. Bars, 10 µm (A,B,D-F), 1 µm (C).