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Fig. 6. Immunofluorescence analysis of COS7 cells transfected with full length envoplakin (E-full) or periplakin (P-full) or constructs lacking the rod domains (envoplakin, E{Delta}Rod; peiplakin, P{Delta}Rod). The cells were fixed without prior extraction (A-C,E,F,H,J-L) or extracted before fixation (D,G,I). Green fluorescence: anti-FLAG (detects tagged envoplakin; A,C-E,G-J,L); anti-HA (detects tagged periplakin; B). Red fluorescence: anti-HA (C,F,G,H,K,L); anti-vimentin (A,B,D,I). Blue fluorescence: nuclei stained with TOTO-3. In D note residual nuclear fluorescence attributable to E{Delta}Rod. Bars, 20 µm (C-H); 5 µm (A,B,I-L).