Fig. 7. Immunofluorescence analysis of COS7 cells transfected with E-1/2N1/2R alone (A-H) or in combination with P-full (K,L) or with P-full alone (I,J). Cells were fixed without prior extraction (A-D, I-K) or extracted with 0.6% saponin before fixation (E-H,L). Green fluorescence: anti-FLAG (to detect tagged envoplakin; A,B,D-F,H,K,L); anti-HA (to detect tagged periplakin; I,J). Red fluorescence: anti-vimentin (A,C-E,G-I,K,L), anti-keratin (J). Boxed areas in A,E are shown at higher magnification in B-D and F-H, respectively. Bars, 10 µm (A-H,L); 5 µm (I-K).