Fig. 3. Fluid shear stress does not cause polarization of clathrin AP-1/2 or microtubules. Endothelial cells were exposed to laminar shear stress as described in the legend to Fig. 2. Cells were then fixed and processed to localize the indicated protein. Arrows indicate regions where caveolin-1 has accumulated and are pointing in the direction of fluid flow. Bar, 20 µm.