Fig. 4. Quantification of caveolin-1 distribution in shear stressed cells. Endothelial cells were either exposed to the indicated laminar shear stress force for 24 hours (right panel) or to a constant force of 20 dynes/cm² for different times (left panel). They were then fixed and processed for localization of caveolin-1. Representative cells were picked and scored according to whether the caveolin-1 staining was principally in one of five regions of the cell (top left), designated A, B, C, D or E. Region A corresponded to the most upstream region of the cell. The percent of cells in each group (ordinate) as a function of time or force (abscissa) was then plotted.