Fig. 6. Caveolin-1 mRNA and protein do not change in stressed cells. Endothelial cells were exposed to a shear stress of 20 dynes/cm² for 1, 3, 6, 12 or 24 hours. Cells were then processed either for immunoblotting of caveolin-1 (A) or RT/PCR analysis of caveolin mRNA (B) as described. The appropriate band for caveolin-1 is indicated in each gel. The mRNA for GAPDH (3-phosphate glyceraldehyde dehydrogenase) was used as a load control.