Fig. 1. Ectopic Pax3 expression induces cell aggregation and epithelioid morphologic changes in Saos-2 and Rh30 cells. Saos-2 cells were infected with adenoviruses, which simultaneously encoded GFP, in addition to either ß-galactosidase (Ad-ßgal) or flag-epitope tagged Pax3 (Ad-Pax3^flag) as indicated. At two days postinfection, GFP expression in live cells was assessed by fluorescence microscopy (A, upper panels). Phase-contrast images of the same fields are shown in A, lower panels. (B,C) Phase-contrast images of control infected and Ad-Pax3^flag-infected Saos-2 cells (B) and Rh30 cells (C) at three days postinfection. Bars, 80 µm.