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Fig. 2. Scanning electron microscopic analysis of Pax3-induced morphologic alterations in Saos-2 cells. (A-D) Scanning electron microscopic micrographs of Ad-ßgal control infected (A,B) or Ad-Pax3flag-infected (C,D) Saos-2 cells at three days postinfection. (E,F) Higher magnification views of the dorsal cell surface highlighting the presence of membrane blebbing (arrowheads) in both control (E) and Ad-Pax3flag-infected (F) cells, as well as the presence of cilia (arrow) in Ad-Pax3flag-infected cells.