Fig. 4. Ectopic Pax3 expression induces the formation of epithelial cell-cell junctions in Saos-2 cells. Soas-2 cells were infected with either Ad-ßgal control virus (a,d,f,h) or Ad-Pax3^flag adenovirus (b,c,e,g,i). The junctional staining of pan-cadherin (d,e), ß-catenin (f,g), -catenin (h,i) and cadherin-11 (a-c) were analyzed by indirect immunofluorescence confocal microscopy at three days postinfection. Bars, 20 µm.