Fig. 7. Ectopic Pax3^flag expression induces rearrangement of actin cytoskeleton. Saos-2 cells were infected with either Ad-ßgal control (a-d,i) or Ad-Pax3^flag (e-h,j) adenoviruses. Cytoskeleton architecture was assessed at three days postinfection by examination of the distribution of Factin (a-h) and vinculin (i,j) by fluorescence confocal microscopy. Shown are individual optical sections captured below the level of the nucleus (a,e), at the level of the nucleus (b,f) and at the most apical regions (c,g). Projections of optical sections are shown in d and h. Inset in g is a magnified image of the cell indicated by arrowhead. In control infected cells (i), vinculin localizes to focal adhesions. In Ad-Pax3^flag-infected cells (j), vinculin localizes to sites of cell-cell contact (arrow), at the tips of filopodia and diffusely throughout the cytoplasm. Bars, 20 µm.