Fig. 8. Ectopic Pax3 expression induces formation of an epithelial-type microtubule organization. Saos-2 cells were infected with control (a—d) or Ad-Pax3^flag (e-k) adenoviruses, and microtubule organization was assessed at three days postinfection by indirect immunofluorescence confocal microscopy with antibodies to ß-tubulin. Optical sections at a level below the nucleus (a,e,i), at the level of the nucleus (b,f,j) and through the apical most region (c,g,k) revealed distinct microtubule organization between Ad-ßgal- and Ad-Pax3^flag-infected cells. Images in panels i, j and k are magnified images of e, f and g, respectively. Note the presence of many punctate dots at all levels in Ad-Pax3^flag-infected cells. (d,h) Projections of optical sections from each respective series. Bars, 10 µm.