Fig. 5. Dome formation is under the control of both E-cadherin and ß1 integrin
expressions. Caco-2 cells were plated at 125,000 cells/cm2 on
plastic ([UNK]) or on native LN5-rich ECM (
) in the presence of 10
µg/ml mouse non-specific IgG (
), 10 µg/ml (
) or 2.5µg/ml
(
) anti-ß1-integrin blocking antibody. (A) shows the appearance of
domes as a function of time. Note that anti-ß1-integrin antibody dose
dependently decreases the number of domes formed on ECM substrate as early as
2 days in culture. These perturbations are not correlated with the confluence
rate. (B) shows the confluence rate of Caco-2 cells. The presence of
ß1-integrin antibody or plastic support delays confluence, which is
reached 2 days later than in untreated cells grown on ECM or in the presence
of control IgG antibody.