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Fig. 7. TgMIC7 and TgMIC9 are microneme proteins poorly expressed in tachyzoites. (A) Western blot analysis of lysates from wild-type RH or Prugniaud strain and transgenic RH parasites stably transformed with MIC7Ty or MIC9Ty under the control of the constitutive promoter TUB1. (B) Double IFA by confocal microscopy of transgenic parasites expressing MIC7Ty. MIC7Ty colocalized ({alpha}-Ty, red) with the microneme marker TgMIC3 (green). (C) Double IFA of transgenic parasites expressing MIC9Ty. MIC9Ty (red) colocalized with TgMIC3 (green). (D) In addition to the microneme localization, MIC9Ty (green) partially localized to the rhoptries, as documented by double IFA with {alpha}-ROP2 (red). Bar, 1 µm.