Fig. 8. MIC8 serves as escorter for the non-membrane adhesin TgMIC3. (A) Double IFA analysis by confocal microscopy of parasites transiently transfected with pTMIC8GPI. TgMIC8 covalently linked to a GPI anchor localized perfectly at the plasma membrane of the parasites. TgMIC3 redistributed to the plasma membrane in the transiently transfected parasites, while the protein is perfectly sorted to the micronemes in a vacuole containing non-transfected parasites. (B) Three examples of vacuoles containing parasites transfected with 30 µg of pTMIC8GPI vectors and analyzed 48 hours post-electroporation. MIC8GPI is stained with the -Ty1 (red) and accumulates at the plasma membrane specifically in the zones of contacts between parasites. The -MIC6 is used as microneme marker (green). Bar, 1 µm.