Fig. 5. Meiotic defects in the absence of Pds5. (A) Phase-contrast micrographs of living cells (upper panels) and fluorescence micrographs of DAPI-stained, formaldehyde-fixed cells (lower panels) of sporulating 428h/429h (pds5⁺/pds5⁺) and pds5/pds5 strains are shown, as indicated. Bars, 10 µm. (B) Quantification of the sporulation defect in pds5/pds5 progeny. The percentages of all asci containing four, three, two or one spores, and the overall spore viabilities of 428h/429h (pds5⁺) and pds5/pds5 (pds5) strains are shown, as indicated (error bars represent one standard deviation in the right-hand panel; the mean of three separate measurements is shown).