Fig. 6. Rad21 and Pds5 are present in high molecular weight complexes of similar sizes. The sizes of soluble Rad21-HA and Pds5-HA were estimated in parallel by gel filtration chromatography as described in Materials and Methods and cell extracts from the rad21-HA and pds5-HA strains. Samples of each 0.5 ml Superose 6 column fraction were processed for immunoblotting with an anti-HA antibody. The elution volume (ml) of each fraction is shown above the blots. The positions at which dextran blue (void volume; 2000 kDa) and thyroglobin (670 kDa) migrated are indicated. The remaining smaller size fractions did not contain significant amounts of either HA-tagged protein.